Original ELISA kit quality how to distinguish - Database & Sql Blog Articles

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Shanghai Jinma Experimental Equipment Co., Ltd. is a high-tech enterprise specializing in life science and biotechnology. It specializes in elisa kit, original ELISA kit, elisa kit supplier, chicken ELISA kit, pig ELISA kit, rat. Elisa kit, microbial culture media, serum, biological reagents, standard reference products, antibodies and other products. Shanghai Jinma is the original Elisa kit gold supplier. We provide customers with three points to distinguish the quality of Elisa kit. If you have any research on the Elisa kit identification method, please feel free to give suggestions or comments.
First, the application of the original ELISA kit

Using the genetic site-directed modification technology CRISPR-Cas system, precise localization of gene modifications or knockouts on haploid stem cells can be performed quickly and efficiently, and the treated cells can maintain haploid and pluripotent states. More importantly, this work demonstrates that rat haploid embryonic stem cells also have the ability to "fertilize" sperm and oocytes and produce healthy rats.

Second, how to judge the original ELISA kit test results

1. Measured method: After the substrate was added and the reaction was terminated, the color of the positive hole was visually observed to be deeper than that of the negative control; if it was close to the color of the negative control, it was judged to be negative.

2. The OD value of the sample well is greater than the negative control OD value +2 to 3 SD as the threshold for the positive result.

3. P/N value (positive hole OD value / negative hole OD value) is greater than or equal to 2.1 is positive; P/N value is less than 2.1, but greater than 1.5 is suspicious; P/N is less than 1.5 is negative.

4. Quantitative measurement results The content of the analyte in the sample is calculated from the standard curve.

Third, research on the original ELISA kit shows

The imported rat elisa kit was studied and the level of rat sex hormone binding globulin (SHBG) elisa kit in the specimen was determined by double antibody sandwich method. The purified rat rat sex hormone binding globulin (SHBG) elisa kit is coated with a microplate to prepare a solid phase antibody, and the sex hormone binding globulin (SHBG) elisa reagent is sequentially added to the microwell of the coated monoclonal antibody. The cassette is then combined with an HRP-labeled sex hormone-binding globulin (SHBG) elisa kit antibody to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with the sex hormone binding globulin (SHBG) elisa kit in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of the rat sex hormone binding globulin (SHBG) elisa kit in the sample was calculated from a standard curve.
Shanghai Jinma Research Center recommends that customers choose the original Elisa kit from the following points:

â—ŽWe should try our best to choose regular manufacturers. The products should be reviewed and approved by the corresponding government departments. The reagents should be comprehensively evaluated from sensitivity, specificity, precision, stability, simplicity, safety and economy. â—Ž Sensitivity: There are two layers of meaning, 1) the ability to detect the minimum amount of the substance to be tested; 2) the ability of the reagent to detect positively in a large number of samples. â—Ž Specificity: expressed by the commonly used cross-reaction rate. Substances that contain structural components close to the analyte may cross-react and increase the assay, which may lead to false positives, so cross-reactivity is a key indicator for evaluating its quality. â—Ž Precision: ELISA reagent generally refers to the CV% in the batch, the value should be less than 15%; the quantitative reagent should also examine the linear range. â—Ž Accuracy: Evaluation was carried out by adding a recovery experiment. â—ŽSimpleness: Under the premise of not affecting the first three indicators of the reagent, the less the experiment and the measurement procedure, the better. In the qualitative experiment, the judgment of the result is simple and clear, and the calculation of the quantitative test result should be simple. â—ŽSafety: It means that the reagent is safe and harmless to the operator and the environment. â—Ž Economical: Reagents reduce costs through mass production or technological advancement under the same quality conditions, and the market price is reasonable.

â—ŽReagent evaluation: An authoritative confirmation method and a confirmed sample are required for testing.

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