Spectrophotometric detection of ethylene oxide (EO) residues

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Determination of residual ethylene oxide (EO) by spectrophotometry

$Width="1099" height="853" src="http://i.bosscdn.com/blog/nophoto.gif" /></p><table cellspacing="0" cellpadding="0" width=" 584" border="1"> <tbody> Spectrophotometric Determination of Residual Ethylene Oxide (EO) Keywords: Ethylene Oxide (EO); Spectrophotometer; US Instruments ; UV -1100 1. Test purpose To test the residual amount of ethylene oxide after disinfection of disposable medical equipment by ethylene oxide, to determine whether it meets the national standards. 2. Test principle Ethylene oxide is hydrolyzed to ethylene glycol under acidic conditions, ethylene glycol is oxidized by periodate to form formaldehyde, and formaldehyde reacts with magenta-sulfuric acid test solution to produce a purple-red compound, which can be obtained by colorimetric analysis. The content of ethylene oxide is obtained. 3. Test equipment and reagent pipette, volumetric flask (50ml, 100ml, 1000ml), measuring cylinder, beaker, analytical balance, dropper, spectrophotometer, Nessler colorimetric tube 0.1mol / L hydrochloric acid solution, 5g / L high Iodine solution, 10g/L sodium thiosulfate, 100g/L sodium sulfite solution, magenta-sulfuric acid test solution, ethylene glycol standard stock solution, distilled water 4. Preparation of reagents 0.1mol/L hydrochloric acid solution: Take <u> </u>ml hydrochloric acid diluted to <u> </u> ml 5g / L periodic acid solution: weighed periodic acid <u> </u>g, diluted with water to <u> </u> ml 10g /L sodium thiosulfate: Weighed <u> </u>g sodium thiosulfate, diluted with water to form <u> </u> ml 100g / L sodium sulfite solution: weighed <u> </u>g sodium sulfite Dilute with water to <u> </u> ml Magenta-sulfuric acid test solution: Weigh <u> </u>g basic fuchsin, add <u> </u> ml, dissolve in hot distilled water at 80 Â°C. After cooling, 100 g/mL sodium sulfite solution <u> </u> ml, hydrochloric acid <u> </u> ml was added and placed in the dark for more than 1 h. The test solution should be colorless. If it is found to be reddish, it should be reconstituted. Ethylene glycol standard stock solution: Take an external dry, clean <u> </u> ml volumetric flask, add about </u>ml of water, and weigh accurately. Accurately measure <u> </u> ml of ethylene glycol, quickly add to the bottle, shake well, and accurately weigh. The difference between the two weighings is the weight of the ethylene glycol contained in the solution, diluted with water to the mark, and mixed. The concentration of ethylene glycol is: 5. Preparation of the test solution The preparation of the test solution is carried out by the limit extraction method, and the representative part of the whole sample or sample is taken, and the fragments are cut into pieces of length [u> </u>mm. Weighed <u> </u> 1.0g into a beaker, added 0.1mol / L hydrochloric acid solution <u> </u> ml, and allowed to stand at room temperature for 1 h, as a test solution. 6. Test procedure 6.1 Take 5 Nessler colorimetric tubes, add 0.1mol/L hydrochloric acid <u> </u>ml, and then add <u> </u>ml, <u> </u>ml , <u> </u>ml, <u> </u>ml, <u> </u> ml ethylene glycol standard solution, and then take another Nessler colorimetric tube, accurately add 0.1mol / L Hydrochloric acid solution <u> </u> ml was used as a blank control. 6.2 Add periodic acid solution to each tube, shake it, and let it stand for 1 hour. The sodium thiosulfate solution was then added dropwise until the yellow color appeared to disappear. Then add magenta-sulfuric acid test solution <u> </u> ml, dilute to <u> </u> ml with distilled water, shake well, place at 35 Â° C ~ 37 Â° C for 1 h, measure at 560 nm wavelength Absorbance, blank solution as reference solution, draw absorbance-volume standard curve. 6.3 Accurately measure the test solution 2.0ml in the Nessler colorimetric tube, operate as described in step 2, measure the absorbance, and obtain the corresponding volume from the standard curve. <table cellspacing="0" cellpadding="0" border="1"> <tbody> volume absorbance The average value of the absorbance of the test solution 12 </ tbody> </ table> 7. Calculation results ring 8. The relative content based on the determination results ethane GB 15980-2009, disposable syringes, surgical gauze dressings and ethylene oxide residues in other medical supplies should be less than <u> </ u>. Therefore, if the amount of ethylene oxide remaining in the sample to be tested is less than or equal to <u> </u>, it is unqualified. 9. Reference standard Medical blood transfusion, infusion syringe inspection method (GB/T 14233.1-2008); disposable medical supplies hygiene standards (GB 15980-2009). Keywords: ethylene oxide (EO); spectrophotometer; US instrumentation ; UV-1100 </tbody></table></p> </div> </div> <div class ="tech-detail-share">  <div class="bdsharebuttonbox"> <a href="#" class="bds_qzone" data-cmd="qzone" title="Share Go to the QQ space"></a> <a href="#" class="bds_tsina" data-cmd="tsina" title="Share to Sina Weibo"></a> <a href="#" class ="bds_weixin" data-cmd="weixin" title="Share to WeChat"></a> <span>Share to:</span> </div> <script>window._bd_share_config = { "common": { "bdSnsKey": {}, "bdText": "", "bdMini": "1", "bdMiniList": false, "bdPic": "", "bdStyle": "2", "bdSize": "16" }, "share": {} }; with (document) 0[(getElementsByTagName($

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