ELISA kit analysis of double antibody sandwich method - Database & Sql Blog Articles

Elisa kit analysis of double antibody sandwich ELISA kit breakthrough - double antibody sandwich method: In one test, when the concentration of antigen in the sample is high, superantigen binds to solid phase antibody and enzyme-labeled antibody, and no longer forms Sandwich compound. Similar to the precipitation reaction after the excess of the antigen, the absorbance value of the color reaction and the standard curve of the same light absorption value, such as the method of measuring the frequently read content, the result will be lower than the actual, this phenomenon is called hook effect (hook Effect), because the standard curve reaches the peak, the hook bends.
A variety of existing dog and wolf genes were compared using the human Elisa kit, but modern samples may be unreliable. If multiple identical epitopes are included in different parts of the test molecule, such as the determinants of hepatitis B surface antigen, the same monoclonal antibodies as the ELISA assay kit can also be used for this determination to coat the solid phase and prepare the enzyme conjugate, respectively. . However, problems should be noted in the detection of subtypes.
Another of the antigens detected by the double antibody sandwich method is rheumatoid factor interference. Radiofrequency is an autoantibody, antibody, and antibody binding to various animals. The debate about how dogs are first domesticated has not yet reached a conclusion, and the source of domestic cats has attracted the attention of scientists. The use of radiocarbon dating has traced the age of these bones to a 200-year period of about 5,300 years ago, which is more than 3,000 years before the domestic cats appeared in China.